anti peroxisome proliferator activated receptor alpha  (Novus Biologicals)

Journal: Biomedicines

Article Title: Lipin-1 Drives Browning of White Adipocytes via Promotion of Brown Phenotype Markers

doi: 10.3390/biomedicines13092069

Figure Lengend Snippet: PPARγ activation upregulates Lipin-1, SIRT1, and SRSF10 expression and alters Lipin-1 isoform splicing in 3T3-L1 adipocytes. ( A ) Relative mRNA expression of Lpin1 , Sirt1 , and Srsf10 following PPARγ activation by 5 μM RGZ on Day 8 of differentiation. ( B ) Protein expression of Lipin-1 in RGZ-treated cells compared with untreated controls. ( C ) mRNA expression of Lpin1a and Lpin1b isoforms in the same treatment group. Statistical significance was assessed by Student’s t -test for ( A , B ) and one-way ANOVA followed by Tukey’s post hoc test for ( C ), comparing treated groups to the untreated control. Data are presented as means ± SEM from three independent experiments. * p <  0.05; ** p <  0.01; *** p <  0.001; **** p <  0.0001; ns, not significant.

Article Snippet: Protein detection was carried out using primary antibodies against Lipin-1 (Novus Biologicals, Centennial, CO, USA; Cat. No: NB110-57150), UCP1 (R&D Systems, Minneapolis, MN, USA; Cat. No: MAB-6158), PGC1 alpha (Novus Biologicals, Centennial, CO, USA; Cat. No: NBP1-04676), and PPAR alpha (Novus Biologicals, Centennial, CO, USA; Cat. No: NB600-636).

Techniques: Activation Assay, Expressing, Control

Journal: Biomedicines

Article Title: Lipin-1 Drives Browning of White Adipocytes via Promotion of Brown Phenotype Markers

doi: 10.3390/biomedicines13092069

Figure Lengend Snippet: SIRT1 activation and brown adipocyte marker expression. ( A ) PPARα and ( B ) UCP1 protein levels appeared elevated in 3T3-L1 cells treated with SRT1720 during the differentiation period. Statistical significance was assessed by Student’s t -test, comparing treated groups to the untreated control. Data are presented as means ± SEM from three independent experiments. * p <  0.05; *** p <  0.001.

Article Snippet: Protein detection was carried out using primary antibodies against Lipin-1 (Novus Biologicals, Centennial, CO, USA; Cat. No: NB110-57150), UCP1 (R&D Systems, Minneapolis, MN, USA; Cat. No: MAB-6158), PGC1 alpha (Novus Biologicals, Centennial, CO, USA; Cat. No: NBP1-04676), and PPAR alpha (Novus Biologicals, Centennial, CO, USA; Cat. No: NB600-636).

Techniques: Activation Assay, Marker, Expressing, Control